We notably see that increasing biomass loading, while keeping enzyme loading constant should be favored for both small- ( R 1) does not bring a significant benefit. In such cases, diffusion of cellulases to the available cellulose surface limits the rate of glucose release. ResultsĮxploring the effect of various experimental and structural parameters highlighted the significant role of internal mass transfer as the substrate size increases and/or the enzyme loading decreases. Our model carefully considers the overall quantity of cellulase present in the hydrolysis mixture and explores its interplay with the available accessible cellulose surface. In this study, we used a model based on a set of partial differential equations describing the evolution of the substrate morphology to investigate the interplay between experimental conditions and the physical characteristics of biomass particles as the reaction proceeds. Note that if there is excess substrate (the first part of the graph) the line can be approximated to a straight line.Understanding how the digestibility of lignocellulosic biomass is affected by its morphology is essential to design efficient processes for biomass deconstruction. Any further increase in enzyme concentration has no further effect on the reaction rate. Some enzymes become redundant as they won't have any substrate to bind. However, there is a limit as eventually there will be more enzyme molecules that substrate. As a result enzyme-substrate complexes form more quickly and the rate of reaction increases. Increasing the amount of enzyme also increases the frequency of with which the enzyme and substrate collide. Any further increase in substrate concentration has no further effect on the reaction rate. However, there is a limit as eventually there all the enzyme active sites are already occupied with substrate - the enzyme '''active sites become saturated'''. Increasing substrate concentration increases the frequency with which the enzyme and substrate collide.
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